Human SOX17 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF1924
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Asp177-Val414
Accession # Q9H6I2
Specificity
Detects human SOX17 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SOX17 Biotinylated Antibody
SOX17 in iBJ6 Cell Line.
SOX17 was detected in immersion fixed iBJ6, an induced pluripotent stem (iPS) cell line, differentiated to endoderm using Goat Anti-Human SOX17 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1924) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human SOX17 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human BG01V embryonic stem cells and iBJ6 induced pluripotent stem cell line differentiated to endoderm
Sample: Immersion fixed human BG01V embryonic stem cells and iBJ6 induced pluripotent stem cell line differentiated to endoderm
Western Blot
0.1 µg/mL
Sample: Recombinant Human SOX17
Sample: Recombinant Human SOX17
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX17
SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2). In Western blot, BAF1924 detects a 60 kDa band of endogenous SOX17 which is higher than predicted molecular weight but consistent with the current literature (3).
References
- Kanai-Azuma, M. et al. (2002) Development 129:2367.
- Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
- Sohn, J. et al. (2006) J. Neuroscience 26:9722.
Long Name
Transcription Factor SOX17
Alternate Names
FLJ22252, SRY (sex determining region Y)-box 17, SRY-related HMG-box transcription factor SOX17, transcription factor SOX-17, VUR3
Entrez Gene IDs
64321 (Human)
Gene Symbol
SOX17
UniProt
Additional SOX17 Products
Product Documents for Human SOX17 Biotinylated Antibody
Product Specific Notices for Human SOX17 Biotinylated Antibody
For research use only
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