Human SOX6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB77591

R&D Systems, part of Bio-Techne
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MAB77591-100
MAB77591-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 667162

View all SOX6 Antibodies »

Product Summary for Human SOX6 Antibody

Immunogen

E. coli-derived recombinant human SOX6
Met1-Leu339
Accession # P35712

Specificity

Detects human SOX6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SOX5 or rhSOX13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SOX6 Antibody

Detection of SOX6 in Human Liver Cancer.

SOX6 was detected in immersion fixed paraffin-embedded sections of Human Liver Cancer using Mouse Anti-Human SOX6 Monoclonal Antibody (Catalog # MAB77591) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human SOX6 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Liver Cancer
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX6

SOX6 is a 92 kDa member of the Sox [Sry-related high mobility group (HMG) box] DNA binding protein family, and initially was isolated from an adult testis cDNA library. Human SOX6 is 828 amino acids (aa) in length. Aa 184-262 constitute a coiled-coil region. Aa 219-261, 280-285, and 313-317 make up a Glu-rich and two poly-Ala regions, respectively. Also, there are two additional isoforms for SOX6. Isoform 2 is formed by the deletion of aa 327-367 found in isoform 1, and isoform 3 is formed by the deletion of aa 579-598 found in isoform 1. Finally, aa 620-683 make up the SOX-TCF-HMG-box region. Human SOX6 shares 97% aa identity with mouse SOX6. Previous studies have suggested that SOX6 plays a role in the development of the central nervous system (CNS) and chondrogenesis. Another study, however, revealed that the mutant pIOOH allele, which is located on the same chromosome as SOX6, develops myopathy and an atrioventricular (AV) heart block, a cardiac conduction defect that is a main cause of death in human cardiac myopathies. Electronmicroscopic evaluation of the mutant cardiac and skeletal muscle demonstrated significant change in ultrastructure. Thus, the phenotype of the pIOOH mutation suggests that the SOX6 protein also may be involved in maintaining normal physiological functions of muscle tissue, including the heart. In addition genome-wide association studies have found that the SOX6 gene plays an important role in the coregulation of obesity and osteoporosis. Moreover, SOX6 has been shown to be a transcriptional factor that is specifically expressed in the developing nervous system and in the early stages of chondrogenesis in mouse embryos, and it has been revealed that SOX6 was expressed in glioma tissues, but not in normal adult brain tissue.

Long Name

SRY-related HMG-box 6

Alternate Names

SOXD

Entrez Gene IDs

55553 (Human); 20679 (Mouse); 293165 (Rat)

Gene Symbol

SOX6

UniProt

P35712 (Human)

Additional SOX6 Products

Product Documents for Human SOX6 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SOX6 Antibody

For research use only

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