Human SPRY3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5735

R&D Systems, part of Bio-Techne
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AF5735
AF5735-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all SPRY3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SPRY3
Met1-Glu142
Accession # O43610

Specificity

Detects endogenous human SPRY3 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human SPRY3 Antibody

Detection of Human SPRY3 antibody by Western Blot.

Detection of Human SPRY3 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human SPRY3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5735) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRY3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SPRY3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPRY3

SPRY3 (sprouty homolog 3) is a member of the sprouty family of proteins. SPRY3 appears to modulate Ras/MAPK pathway signaling following RTK activation. Human SPRY3 is 288 amino acids (aa) in length and contains one SH2-binding domain (aa 25‑30), a PEST sequence (aa 82‑93), and a Cys-rich domain (aa 147‑270). SPRY3 forms homo- and hetero-oligomers with other SPRY family molecules. It also undergoes phosphorylation, ubiquitination and palmitoylation, the latter of which induces SPRY3 to associate with cell membranes.

Long Name

Sprouty Homolog 3

Alternate Names

Antagonist Of FGF Signaling, HSPRY3, Protein Sprouty Homolog 3, sprouty (Drosophila) homolog 2, Sprouty Homolog 3 (Drosophila), spry-3

Entrez Gene IDs

10251 (Human); 236576 (Mouse)

Gene Symbol

SPRY3

UniProt

O43610

Additional SPRY3 Products

Product Documents for Human SPRY3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SPRY3 Antibody

For research use only

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