Human ST8 alpha-2,8‑Sialyltransferase 8A/ST8SIA1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6716

R&D Systems, part of Bio-Techne
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AF6716
AF6716-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ST8 alpha-2,8‑Sialyltransferase 8A/ST8SIA1
Tyr49-Ser356
Accession # Q92185

Specificity

Detects human ST8 alpha-2,8‑Sialyltransferase 8A/ST8SIA1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ST8 alpha-2,8‑Sialyltransferase 8A/ST8SIA1 Antibody

Detection of Human ST8 a-2,8-Sialyltransferase 8A/ ST8SIA1 antibody by Western Blot.

Detection of Human ST8 alpha‑2,8‑Sialyltransferase 8A/ ST8SIA1 by Western Blot.

Western blot shows lysates of Hs 294T human melanoma cell line, SK-Mel-28 human malignant melanoma cell line, A375 human melanoma cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ST8 a-2,8-Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST8 a-2,8-Sialyltransferase 8A/ST8SIA1 at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ST8 a-2,8-Sialyltransferase 8A/ST8SIA1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

ST8 alpha‑2,8‑Sialyltransferase 8A/ST8SIA1 in Human Breast Cancer Tissue.

ST8 a-2,8-Sialyltransferase 8A/ST8SIA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human ST8 a-2,8-Sialyltransferase 8A/ST8SIA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6716) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ST8 alpha-2,8‑Sialyltransferase 8A/ST8SIA1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Western Blot

2 µg/mL
Sample: Hs 294T human melanoma cell line, SK‑Mel‑28 human malignant melanoma cell line, A375 human melanoma cell line, and Bowes human melanoma cell line

Reviewed Applications

Read 1 review rated 4 using AF6716 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1

Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues and are particularly prevalent on neuronal cells (1). Ganglioside GD3 is involved in cell adhesion and the growth of cultured malignant cells (2). ST8SIA1 is a sialyltransferase that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 (NeuNAc alpha2‑3Gal beta1‑4Glc‑Cer) to produce GD3 (NeuNAc alpha2‑8NeuNAc alpha2‑3Gal beta1‑4Glc‑Cer) and GT3 (NeuNAc alpha2‑8NeuNAc alpha2‑8NeuNAc alpha2‑3Gal beta1‑4Glc‑Cer) in a successive manner (3); therefore the enzyme has both GD3 and GT3 synthase activity (4). ST8SIA1 is mainly expressed in adult and fetal brain, and its expression is enhanced in melanoma cell lines (3, 4, 5). Like most known glycosyltransferases, ST8SIA1 is predicted as a type II transmembrane protein with a short N‑terminal cytoplasmic domain and a single-pass transmembrane domain followed by an enzymatic domain in the lumen of the Golgi apparatus. However, recently GD3 synthase activity was demonstrated at the surface of epithelial and melanoma cells, suggesting glycosphingolipid synthesis may occur at the cell membrane (6). Recombinant ST8SIA1 also showed activity on fetuin from fetal calf serum, when measured using a phosphatase-coupled method (7).

References

  1. Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
  2. Cheresh, D.A. et al. (1986) J. Cell. Biol. 102:688.
  3. Nakayama, J. et al. (1986) J. Biol. Chem. 271:3684.
  4. Nara, K. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:7952.
  5. Haraguchi, M. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:10455.
  6. Crespo, P.M. et al. (2010) J. Biol. Chem. 285:29179.
  7. Wu, Z.L. et al. (2010) Glycobiology doi: 10.1093/glycob/cwq187.

Long Name

ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1

Alternate Names

GD3S, SIAT8A, ST8SiaI

Entrez Gene IDs

6489 (Human); 20449 (Mouse); 25280 (Rat)

Gene Symbol

ST8SIA1

UniProt

Q92185

Additional ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1 Products

Product Documents for Human ST8 alpha-2,8‑Sialyltransferase 8A/ST8SIA1 Antibody

Product Datasheets

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Product Specific Notices for Human ST8 alpha-2,8‑Sialyltransferase 8A/ST8SIA1 Antibody

For research use only

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