Human STAT2 Antibody
R&D Systems, part of Bio-Techne | Catalog # PAF-ST2
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Knockout Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human STAT2
Gln679-Phe851
Accession # P52630
Gln679-Phe851
Accession # P52630
Specificity
Detects human STAT2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human STAT2 Antibody
Western Blot Shows Human STAT2 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human STAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST2) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT2 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human STAT2 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa cell line, STAT5b KO HeLa cell line, and STAT6 KO HeLa cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human STAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST2) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT2 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human STAT2 Antibody
Application
Recommended Usage
Immunoprecipitation
3 µg/106 cells
Sample: WS-1 human fetal skin fibroblast cell line, see our available Western blot detection antibodies
Sample: WS-1 human fetal skin fibroblast cell line, see our available Western blot detection antibodies
Knockout Validated
STAT2
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
STAT2 knockout HeLa cell line.
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 1 mg/mL in deionized water.
Formulation
Lyophilized from a 0.2 μm sterile-filtered solution in phosphate-buffered saline (PBS) with 5% trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT2
Long Name
Signal Transducer and Activator of Transcription 2
Alternate Names
interferon alpha induced transcriptional activator, ISGF-3, MGC59816, p113, signal transducer and activator of transcription 2, signal transducer and activator of transcription 2, 113kD, signal transducer and activator of transcription 2, 113kDa, STAT113
Gene Symbol
STAT2
UniProt
Additional STAT2 Products
Product Documents for Human STAT2 Antibody
Product Specific Notices for Human STAT2 Antibody
For research use only
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