Human STEAP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB55871
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2959F
Product Summary for Human STEAP1 Antibody
Immunogen
E. coli-derived recombinant human STEAP1
Met1-Trp71
Accession # Q9UHE8
Met1-Trp71
Accession # Q9UHE8
Specificity
Detects human STEAP1 in direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human STEAP1 Antibody
Detection of STEAP1 in HEK293 cells by Flow Cytometry.
HEK293 cells transfected with Human STEAP-1 and eGFP (A) vs irrelevant and eGFP (B) were stained with Rabbit Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB55871) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.
Detection of Human STEAP1 by Western Blot.
Western blot shows lysates of Human prostate cancer and LNCaP. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB55871) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STEAP1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human STEAP1 by Simple WesternTM.
Simple Western lane view shows lysates of LNCaP human prostate cancer cells, loaded at 0.2 mg/mL. A specific band was detected for STEAP1 at approximately 39 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human STEAP1 Monoclonal Antibody (Catalog # MAB55871). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.Applications for Human STEAP1 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 cells transfected with Human STEAP1 and eGFP vs irrelevant
Sample: HEK293 cells transfected with Human STEAP1 and eGFP vs irrelevant
Simple Western
10 µg/mL
Sample: LNCaP human prostate cancer cells
Sample: LNCaP human prostate cancer cells
Western Blot
0.5 µg/mL
Sample: Human prostate cancer and LNCaP
Sample: Human prostate cancer and LNCaP
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STEAP1
Long Name
Six Transmembrane Epithelial Antigen of the Prostate 1
Alternate Names
PRSS24
Gene Symbol
STEAP1
UniProt
Additional STEAP1 Products
Product Documents for Human STEAP1 Antibody
Product Specific Notices for Human STEAP1 Antibody
For research use only
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