Human Survivin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB886
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Intracellular Staining by Flow Cytometry
Cited:
CyTof, Flow Cytometry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 91630
Product Specifications
Immunogen
E. coli-derived recombinant human Survivin
Met1-Asp142
Accession # O15392
Met1-Asp142
Accession # O15392
Specificity
Detects human Survivin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Survivin Antibody
Detection of Survivin in Human PBMC lymphocytes by Flow Cytometry.
Human peripheral blood mononuclear cells were labeled with Human Survivin Monoclonal Antibody (Catalog # MAB886, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with saponin.Applications for Human Survivin Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cell lymphocytes
Sample: Human peripheral blood mononuclear cell lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB886 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Survivin
Survivin is a member of the inhibitor of apoptosis (IAP) family and can inhibit apoptosis induced by a variety of factors. It is expressed in most human cancers but not in normal adult tissues. Survivin is expressed in a cell cycle-dependent manner and associates with the mitotic apparatus.
Additional Survivin Products
Product Documents for Human Survivin Antibody
Product Specific Notices for Human Survivin Antibody
For research use only
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