Human Survivin PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC6472P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 91630
Product Specifications
Immunogen
E. coli-derived recombinant human Survivin
Met1-Asp142
Accession # O15392
Met1-Asp142
Accession # O15392
Specificity
Detects human Survivin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Survivin PE-conjugated Antibody
Detection of Survivin in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human Survivin PE-conjugated Monoclonal Antibody (Catalog # IC6472P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human Survivin PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood lymphocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood lymphocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Survivin
Survivin is a member of the Inhibitor of Apoptosis (IAP) family and can inhibit apoptosis induced by a variety of factors. It is expressed in most human cancers but not in normal adult tissues. Survivin is expressed in a cell cycle-dependent manner and associates with the mitotic apparatus.
Additional Survivin Products
Product Documents for Human Survivin PE-conjugated Antibody
Product Specific Notices for Human Survivin PE-conjugated Antibody
For research use only
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