Western blot shows lysates of SK-BR-3 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB90562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for SUSD2 at approximately 110 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SUSD2 in Human Breast Cancer Tissue.

Sushi Domain Containing 2 (SUSD2) was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB90562) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of SUSD2 in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human SUSD2 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB90562) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins.

Applications for Human SUSD2 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human SUSD2 and eGFP

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Western Blot

1 µg/mL
Sample: SK‑BR‑3 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SUSD2

Sushi domain containing 2, or SUSD2, is a type I transmembrane protein of 822 amino acids containing functional domains inherent to adhesion molecules. SUSD2 has been described as a novel marker of human endometrial mesenchymal stem-like cells and it has been used for their prospective Isolation. As a transmembrane receptor, SUSD2 has been proposed to interact with Galectin-1 and to be the receptor for C10ORF99, a novel potential cytokine suggested to inhibits colon cancer cell growth through inducing G1 arrest. There is evidence that SUSD2 may play a role in breast tumorigenesis.

Long Name

Sushi Domain Containing 2

Alternate Names

BK65A6.2

Entrez Gene IDs

56241 (Human); 71733 (Mouse); 294335 (Rat)

Gene Symbol

SUSD2

UniProt

Q9UGT4

Additional SUSD2 Products

Product Documents for Human SUSD2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SUSD2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human SUSD2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB90562

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SUSD2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SUSD2 Antibody

Detection of SUSD2 by Western Blot.