Human T-bet/TBX21 Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC5385F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Canine
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 525803
Product Specifications
Immunogen
E. coli-derived recombinant human T-bet
Glu326-Asn535
Accession # Q9UL17
Glu326-Asn535
Accession # Q9UL17
Specificity
Detects human T-bet.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human T-bet/TBX21 Fluorescein-conjugated Antibody
Detection of T‑bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry.
Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human T-bet/TBX21 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC5385F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human T-bet/TBX21 Fluorescein-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Jurkat human acute T cell leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: T-bet/TBX21
T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.
Long Name
T-box transcription factor TBX21
Alternate Names
T-PET, Tbet, TBLYM, TBX21
Gene Symbol
TBX21
UniProt
Additional T-bet/TBX21 Products
Product Documents for Human T-bet/TBX21 Fluorescein-conjugated Antibody
Product Specific Notices for Human T-bet/TBX21 Fluorescein-conjugated Antibody
For research use only
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