Human TAZ/WWTR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7210
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 672027
Product Specifications
Immunogen
E. coli-derived recombinant human TAZ/WWTR1
Met267-Leu400
Accession # Q9GZV5
Met267-Leu400
Accession # Q9GZV5
Specificity
Detects human TAZ/WWTR1 in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human (rh) YAP or rhYes is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human TAZ/WWTR1 Antibody
Detection of Human TAZ/WWTR1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TAZ/WWTR1 Monoclonal Antibody (Catalog # MAB7210) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TAZ/WWTR1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TAZ/WWTR1 in BG01V Human Embryonic Stem Cells.
TAZ/WWTR1 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TAZ/WWTR1 Monoclonal Antibody (Catalog # MAB7210) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human TAZ/WWTR1 by Western Blot
Downregulation of CRB3 confers CSC traits on breast cancer cells through the TAZ/ beta-catenin cascade. (a) Western blot detecting the expressions of AMOT, TAZ and beta-catenin. (b) CoIP/western blot analysis showing endogenous AMOT bound to TAZ. (c) Fluorescence-activated cell sorting of CD44high/CD24low population. (d) Western blot of TAZ and stem cell markers. (e, g) Representative images and quantification of formed mammospheres. (f) Western blot of beta-catenin and stem cell markers. XAV939 was used to inhibit beta-catenin. (h) Flow cytometry analysis of the stem cell marker ALDH and the CD44high/CD24low population. All data are presented as mean±s.e.m. and statistical significance was calculated using a two-tailed t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28436991), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human TAZ/WWTR1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and A431 human epithelial carcinoma cell line
Sample: Immersion fixed BG01V human embryonic stem cells and A431 human epithelial carcinoma cell line
Western Blot
2 µg/mL
Sample: A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB7210 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TAZ/WWTR1
TAZ (Transcriptional co-Activatorr with PDZ-binding motif; also WWTR1) is a 50-55 kDa protein that is related to YAP65 TEF-1 interacting protein. It is a widely expressed transcriptional coactivator, and should not be confused with tafazzin/Taz, an enzyme associated with lipid metabolism. TAZ influences the nuclear transport of SMAD-2, -3 and -4, and in the nucleus, TAZ is known to interact with transcription factors such as TFF1, Pax8, NKX2-1 and TEADS, serving as a scaffold for transcriptional activation complexes. Human TAZ is 400 amino acids (aa) in length. It contains one WW domain (aa 124-157) that binds to PPXY motifs, a coiled-coil region (aa 225-259), and a PDZ binding domain (aa 394-400). There are at least four utilized phosphorylation sites. When phosphorylated on Ser89, TAZ preferentially bind to 14-3-3 proteins, promoting its retention in the cytoplasm. Over aa 267-400, human TAZ shares 88% aa identity with mouse TAZ.
Long Name
Transcriptional Coactivator with PDZ-binding Motif
Alternate Names
WWTR1
Gene Symbol
WWTR1
UniProt
Additional TAZ/WWTR1 Products
Product Documents for Human TAZ/WWTR1 Antibody
Product Specific Notices for Human TAZ/WWTR1 Antibody
For research use only
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