Human TBK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF9934
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TBK-1
Met1-Gly121
Accession # Q9UHD2
Met1-Gly121
Accession # Q9UHD2
Specificity
Detects human TBK-1 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human TBK1 Antibody
TBK1 in Human Prostate Cancer Tissue.
TBK1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human TBK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9934) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human TBK1 by Simple WesternTM.
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cells, and HeLa human cervical epithelial carcinoma cells, loaded at 0.2 mg/mL. A specific band was detected for TBK1 at approximately 90 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human TBK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9934) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human TBK1 Antibody
Application
Recommended Usage
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue
Simple Western
20 µg/mL
Sample: Daudi human Burkitt's lymphoma cells, and HeLa human cervical epithelial carcinoma cells
Sample: Daudi human Burkitt's lymphoma cells, and HeLa human cervical epithelial carcinoma cells
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TBK1
Long Name
TANK Binding Kinase 1
Alternate Names
FTDALS4, NAK, NFKB-Activating Kinase
Gene Symbol
TBK1
UniProt
Additional TBK1 Products
Product Documents for Human TBK1 Antibody
Product Specific Notices for Human TBK1 Antibody
For research use only
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