Human TCF7/TCF1 Antibody

Detection of Human TCF7/TCF1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TCF7/TCF1 at approximately 60, 40, and 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TCF7/TCF1 in HCT‑116 Human Cell Line.

TCF7/TCF1 was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TCF7/TCF1 in Human Thymus.

TCF7/TCF1 was detected in immersion fixed paraffin-embedded sections of human thymus using Goat Anti-Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.