Human Tenascin X Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6999
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Tenascin X isoform 2
Met1-Gly673
Accession # AAH33740
Met1-Gly673
Accession # AAH33740
Specificity
Detects recombinant human Tenascin X isoform 2 in direct ELISAs and human Tenascin X in Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Tenascin R is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Tenascin X Antibody
Detection of Human Tenascin X by Western Blot.
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tenascin X Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6999) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tenascin X at approximately 470 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Tenascin X in Human Lung Mesothelioma.
Tenascin X was detected in immersion fixed paraffin-embedded sections of human lung mesothelioma using Sheep Anti-Human Tenascin X Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6999) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Tenascin X by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Tenascin X at approximately 400 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Tenascin X Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6999) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.Applications for Human Tenascin X Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung mesothelioma
Sample: Immersion fixed paraffin-embedded sections of human lung mesothelioma
Simple Western
50 µg/mL
Sample: Human heart tissue
Sample: Human heart tissue
Western Blot
1 µg/mL
Sample: Human heart tissue
Sample: Human heart tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tenascin X
Tenascin-XB2 (TN-XB2; also TN-XB-Short/S and tenascin-X isoform 2) is a 73-75 kDa member of the tenascin family of extracellular matrix molecules. It is expressed by adrenal gland, and represents the use of an intronic promoter that is tied to an internal start site within the TN-XB gene. A duplication of the TN-XB2 region has created a gene called TN-XA that codes for the same ORF but is not translated. Although TN-XB2 has been reported to bind to tropoelastin, plus collagens I, III and V, other studies using soluble fragments of TN-XB report only tropoelastin interaction. Human TN-XB2 is 673 amino acids (aa) in length. It contains four fibronectin type III repeats (aa 82-442), and one C-terminal fibrinogen-like globular region (aa 449-664). Antibodies to TN-XB2 recognize full-length 465 kDA Tenascin X (TN-XB), 75 kDa and 140-150 kDa C-terminal fragments. Although human TN-XB2 shares 84% aa identity with full-length mouse TN-XB in its C-terminus, there does not appear to be an equivalent alternative start site in the mouse gene.
Alternate Names
HXBL, TNX, TNXB, TNXBS, XB, XB-S
Gene Symbol
TNXB
UniProt
Additional Tenascin X Products
Product Documents for Human Tenascin X Antibody
Product Specific Notices for Human Tenascin X Antibody
For research use only
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