Human TGF-beta RII Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-241-NA
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat, Feline, Rabbit
Applications
Validated:
CyTOF-ready, ELISA Capture (Matched Antibody Pair), Flow Cytometry, Immunohistochemistry, Neutralization, Western Blot
Cited:
Bioassay, Blocking, Cell Culture, CyTof, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF-beta RII
Ile24-Asp159
Accession # P37173.2
Ile24-Asp159
Accession # P37173.2
Specificity
Detects human TGF-beta RII in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human TGF-beta RII Antibody
Detection of Human TGF‑ beta RII by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for TGF-beta RII at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TGF-beta RII in Human Lymphocytes by Flow Cytometry.
Human blood-derived lymphocytes were labeled with Goat Anti-Human TGF-beta RII Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF-241-NA, filled histogram) or control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108).
TGF-beta RII in Human Pituitary.
TGF-beta RII was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human TGF-beta RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-241-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human TGF-beta RII Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
1 µg/106 cells
Sample: Human blood-derived lymphocytes
Sample: Human blood-derived lymphocytes
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pituitary
Sample: Immersion fixed paraffin-embedded sections of human pituitary
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, HT1080 human fibrosarcoma cell line, and DU145 human prostate carcinoma cell line
Neutralization
Measured by its ability to neutralize TGF‑ beta1 inhibition of IL‑4-dependent proliferation in the TF‑1 human erythroleukemic cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 5‑20 µg/mL in the presence of 0.04 ng/mL Recombinant Human TGF‑ beta1 and 5 ng/mL Recombinant Human IL‑4.
Human TGF-beta RII Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RII
TGF-beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF-beta RII interacts with TGF-beta RI to form the heteromeric signaling complex thattransduces TGF beta signals. A splice variant of the type II receptor, TGF-beta RIIb, containing a 25 amino acid residue insertion near the Nterminus of the mature protein has also been described.
Long Name
Transforming Growth Factor beta Receptor II
Alternate Names
TGF-bRII, TGFbetaRII, TGFBR2
Gene Symbol
TGFBR2
UniProt
Additional TGF-beta RII Products
Product Documents for Human TGF-beta RII Antibody
Product Specific Notices for Human TGF-beta RII Antibody
For research use only
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