Human Tiki2/TRABD2B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1600
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Human embryonic kidney cell line HEK293-derived transfected with human Tiki2/TRABD2B
Accession # A6NFA1
Accession # A6NFA1
Specificity
Detects human Tiki2/TRABD2B in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Tiki2/TRABD2B Antibody
Detection of Human Tiki2/TRABD2B by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human Tiki2/TRABD2B. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Tiki2/TRABD2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1600) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tiki2/TRABD2B at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Tiki2/TRABD2B in HEK293 Human Cell Line Transfected with Human Tiki2/TRABD2B by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either human Tiki2/ TRABD2B (filled histogram) or irrelevant transfectants (open histogram) was stained with Sheep Anti-Human Tiki2/TRABD2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1600), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). View our protocol for Staining Membrane-associated Proteins.Applications for Human Tiki2/TRABD2B Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human Tiki2/TRABD2B
Sample: HEK293 human embryonic kidney cell line transfected with human Tiki2/TRABD2B
Western Blot
0.5 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human Tiki2/TRABD2B
Sample: HEK293 human embryonic kidney cell line transfected with human Tiki2/TRABD2B
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tiki2/TRABD2B
Alternate Names
HKAT, TRABD2B
Gene Symbol
TRABD2B
UniProt
Additional Tiki2/TRABD2B Products
Product Documents for Human Tiki2/TRABD2B Antibody
Product Specific Notices for Human Tiki2/TRABD2B Antibody
For research use only
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