Human TMEM59 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9118
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 816004
Product Specifications
Immunogen
E.coli-derived recombinant human TMEM59
Met1-Asn220
Accession # Q9BXS4
Met1-Asn220
Accession # Q9BXS4
Specificity
Detects human TMEM59 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TMEM59 Antibody
TMEM59 in HeLa Human Cell Line.
TMEM59 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human TMEM59 Monoclonal Antibody (Catalog # MAB9118) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human TMEM59 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TMEM59
TMEM59 is a 324 aa protein with a 21 amino acid transmembrane domain which localizes to the Golgi compartment where it modulates the O-glycosylation and complex N-glycosylation maturation steps of several proteins including APP, BACE1, SEAP and PRNP. It may retain APP in the Golgi and inhibits amyloid beta generation as well APP cleavage by alpha and beta secretases. TMEM59 also contains a 19 aa peptide that acts as a regulator of autophagy in response to bacterial infection by promoting LC3 activation through interaction with ATG16L1, targeting its own endosomal compartment to lysosomes in response to aggregation during S.areus infection.
References
- Ullrich S, et al, J Biol Chem. 2010 Jul 2;285(27):20664
- Boada-Romero E, et al, EMBO J. 2013 Feb 20;32(4):566
Long Name
Transmembrane Protein 59
Alternate Names
C1orf8, DCF1, HSPC001, PRO195, UNQ169
Gene Symbol
TMEM59
UniProt
Additional TMEM59 Products
Product Documents for Human TMEM59 Antibody
Product Specific Notices for Human TMEM59 Antibody
For research use only
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