Human TMPRSS9 MAb (Clone 1042528)
R&D Systems, part of Bio-Techne | Catalog # MAB5688
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1042528
Product Specifications
Immunogen
E. coli-derived recombinant human TMPRSS9
Glu190-Gln490
Accession # Q7Z410
Glu190-Gln490
Accession # Q7Z410
Specificity
Detects human TMPRSS9 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TMPRSS9 MAb (Clone 1042528)
TMPRSS9 in SARS-CoV-2 infected Human lung.
TMPRSS9 was detected in immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung using Mouse Anti-Human TMPRSS9 Monoclonal Antibody (Catalog # MAB5688) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013) . Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to immunoreactive profiles scattered throughout the tissue. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human TMPRSS9 MAb (Clone 1042528)
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung
Sample: Immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung
Reviewed Applications
Read 1 review rated 5 using MAB5688 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TMPRSS9
TMPRSS9 (Transmembrane protease, Serine 9; also Polyserine protease 1) is a 116-120 kDa (predicted) member of the TTSP family of membrane-bound serine proteases. It is expressed in fetal tissue, and converts pro-uPA into its active form. Human TMPRSS9 is a 1059 type II transmembrane (TM) protein. It has a 29 aa N-terminal cytoplasmic region and a 1008 aa extracellular domain (ECD) (aa 51-1059). The ECD contains one LDLR region (aa 153-190), followed by three Peptidase S1 domains (aa 203-436, 504-736, and 827-1058), the first two of which are catalytically active. Proteolytic cleavage can generate a soluble 34 kDa Serase 1 (aa 203-503), a 35 kDa Serase 2 (aa 504-826) and an inactive 25 kDa Serase 3 (aa 827-1059). Alternatively, truncated, TM forms of TMPRSS9 such as a 56 kDa TM isotype containing only the Serase 1 domain, or a 91 kDa (predicted) form containing the Serase 1, 2 and 3 domains may exist. Two potential splice forms are reported. One shows an insertion of 34 aa after Lys90 accompanied by a seven aa substitution for aa 491-1059. A second isoform shows a 34 aa substitution for aa 822-1059. Over aa 190-490, human TMPRSS9 shares 79% aa identity with mouse TMPRSS9.
Long Name
Transmembrane protease, serine 9
Alternate Names
Polyserase-1, Polyserase-I
Gene Symbol
TMPRSS9
UniProt
Additional TMPRSS9 Products
Product Documents for Human TMPRSS9 MAb (Clone 1042528)
Product Specific Notices for Human TMPRSS9 MAb (Clone 1042528)
For research use only
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