Detects human TNF RII/TNFRSF1B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rhRANK, rhTNF RI, or recombinant mouse TNF RII is observed. Neutralizes the biological effect of rhTNF RII but not rhTNF RI.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TNF RII/TNFRSF1B Antibody

Detection of TNF RII/TNFRSF1B in Human Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB226, filled histogram) or isotype control antibody (MAB003, open histogram) followed by anti-mouse IgG PE-conjugated secondary antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

TNF RII/TNFRSF1B in Human PBMCs.

TNF RII/TNFRSF1B was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB226) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TNF RII/TNFRSF1B Inhibition of TNF-alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody.

Recombinant Human TNF RII/TNFRSF1B inhibits Recombinant Human TNF-a (210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/ TNFRSF1B (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RII/ TNFRSF1B Monoclonal Antibody (Catalog # MAB226). The ND₅₀ is typically 0.5-1.5 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Detection of Human TNF RII/TNFRSF1B by Immunohistochemistry

Representative immunohistochemical staining for TNFR1 and TNFR2 in the kidneys.(A) Images were captured at 200× (a, b, c, d, i, j, k, l) and 100× (e, f, g, h, m, n, o, p) magnification. Images show the negative controls in the glomeruli (a, i) and tubulointerstitium (e, m) and TNFR1 and TNFR2 immunostaining in the glomeruli (b, j) and tubulointerstitium (f, n) of the normal kidneys, respectively. TNFR1 and TNFR2 immunostaining is shown in the glomeruli (c, k) and tubulointerstitium (g, o) of the kidneys from selected IgAN patients who had levels of serum TNFR2 that ranked in the lowest 10 [low group (LG)]. TNFR1 and TNFR2 immunostaining in the glomeruli (d, l) and tubulointerstitium (h, p) of the kidneys from selected IgAN patients who had levels of serum TNFR2 that ranked in the highest 10 [high group (HG)], respectively, are also shown. (B) Percentage of the TNFR1 and TNFR2-positive area in the kidneys were evaluated. Glomerular and tubulointerstitial TNFR expression was elevated significantly in IgAN patients compared with those in control (Ctrl) subjects. The tubulointerstitial TNFR2-positive area was significantly larger in HG than in LG. However, there was no significant difference in the tubulointerstitial TNFR1 and glomerular TNFR areas between LG and HG. * P < 0.01, **P < 0.001, †P < 0.0001. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0122212), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of TNF RII/TNFRSF1B in U937 cells by Flow Cytometry.

U937 cells were stained with Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB226, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.

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Applications for Human TNF RII/TNFRSF1B Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood granulocytes and U937 human histiocytic lymphoma cell line.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Neutralization

Measured by its ability to neutralize TNF RII/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.5-1.5 µg/mL in the presence of 0.3 µg/mL Recombinant Human TNF RII/TNFRSF1B, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RII/TNFRSF1B

TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with highaffinity and is expressed primarily in cells of the immune system.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1B

UniProt

P20333

Additional TNF RII/TNFRSF1B Products

Product Documents for Human TNF RII/TNFRSF1B Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TNF RII/TNFRSF1B Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TNF RII/TNFRSF1B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB226

Key Product Details

Species Reactivity

Validated:

Cited:

Applications