Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 150 ng/mL IFN-a for 48 hours were stained with Mouse Anti-Human TRAIL/TNFSF10 APC-conjugated Monoclonal Antibody (Catalog # FAB687A) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TRAIL/TNFSF10 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with IFN-alpha

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRAIL/TNFSF10

TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.

Long Name

TNF-related Apoptosis-inducing Ligand

Alternate Names

CD253, TNFSF10

Entrez Gene IDs

8743 (Human); 22035 (Mouse); 246775 (Rat)

Gene Symbol

TNFSF10

UniProt

P50591

Additional TRAIL/TNFSF10 Products

Product Documents for Human TRAIL/TNFSF10 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TRAIL/TNFSF10 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human TRAIL/TNFSF10 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB687A

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all TRAIL/TNFSF10 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TRAIL/TNFSF10 APC-conjugated Antibody

Detection of TRAIL/TNFSF10 in Human PBMCs by Flow Cytometry.