Human peripheral blood cells were stained with (A) Mouse Anti-Human TRAILR4 Monoclonal Antibody (Catalog # MAB663R) or (B) Mouse IgG1 isotype control antibody (Catalog # MAB002) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse anti-Human CEACAM-8/CD66b APC-conjugated Monoclonal Antibody (Catalog # FAB4246A). View our protocol for Staining Membrane-associated Proteins.

TRAIL R4/TNFRSF10D Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Human TRAIL R4/TNFRSF10D Antibody.

In the presence of the metabolic inhibitor actinomycin D, Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TEC) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (20 ng/mL) activity elicited by Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (90 ng/mL) is neutralized (green line) by increasing concentrations of Human TRAIL R4/TNFRSF10D Monoclonal Antibody (Catalog # MAB633R). The ND₅₀ is typically 0.3-1.8 µg/mL.

Human TRAILR4/TNFRSF10D ELISA Standard Curve.

Recombinant Human TRAILR4/TNFRSF10D protein was serially diluted 2-fold and captured by Mouse Anti-Human TRAILR4/TNFRSF10D Monoclonal Antibody (Catalog # MAB633R) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human TRAILR4/TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (AF633 ) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY998) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human TRAILR4/TNFRSF10D Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human TRAILR4/TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human TRAIL R4/TNFRSF10D DuoSet ELISA Kit (Catalog # DY633) for convenient development of a sandwich ELISA.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood cells

Neutralization

Measured by its ability to neutralize TRAIL R4/TNFRSF10D-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.3-1.8 µg/mL in the presence of 90 ng/mL Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera, 20 ng/mL of Recombinant Human TRAIL/TNFSF10 and actinomycin D.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAILR4/TNFRSF10D

Human TRAIL R4, also called decoy receptor 2 (DcR2) and TRUNND (TRAIL receptor with a truncated death domain), is a type I, TNF R family transmembrane protein, which is a receptor for TRAIL (APO2 ligand). In the TNF superfamily nomenclature, TRAIL R4 is designated as TNFRSF10D. TRAIL R4 is unique among the TRAIL receptors in that its cytoplasmic domain contains a truncated consensus death domain motif. Binding of TRAIL R4 does not result in an apoptotic signal. Overexpression of TRAIL R4 can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. The human soluble TRAIL R4/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

References

Griffith, T.S. et al. (1998) Curr. Opin. Immunol. 10:559.
Pan, G. et al. (1998) FEBS lett 424:41.
Marsters, S.A. et al. (1997) Cur. Biol. 7:1003.
Degli-Esposti, M.A. et al. (1997) Immunity 7:813.

Long Name

TRAIL Receptor 4

Alternate Names

CD264, DcR2, TNFRSF10D, TRAIL R4

Entrez Gene IDs

8793 (Human)

Gene Symbol

TNFRSF10D

UniProt

Q9UBN6

Additional TRAILR4/TNFRSF10D Products

Product Documents for Human TRAILR4/TNFRSF10D Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human TRAILR4/TNFRSF10D Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TRAILR4/TNFRSF10D Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB633R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human TRAILR4/TNFRSF10D Antibody

Detection of TRAILR4 in Human peripheral blood cells by Flow Cytometry.