Human TRAILR4/TNFRSF10D Antibody
R&D Systems, part of Bio-Techne | Catalog # AF633
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Neutralization, Western Blot
Cited:
Cell Culture, Flow Cytometry, Immunocytochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL R4/TNFRSF10D
Ala56-His211
Accession # Q9UBN6
Ala56-His211
Accession # Q9UBN6
Specificity
Detects human TRAIL R4/TNFRSF10D in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, and rhTRAIL R3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human TRAILR4/TNFRSF10D Antibody
Human TRAILR4/TNFRSF10D ELISA Standard Curve.
Recombinant Human TRAILR4/TNFRSF10D protein was serially diluted 2-fold and captured by Mouse Anti-Human TRAILR4/TNFRSF10D Monoclonal Antibody (MAB633R) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human TRAILR4/TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
TRAIL R4/TNFRSF10D Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Human TRAIL R4/TNFRSF10D Antibody.
In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (5 µg/mL, Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (12 ng/mL) activity elicited by Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL R4/ TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633). The ND50 is typically 0.03-0.1 µg/mL.Applications for Human TRAILR4/TNFRSF10D Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human TRAILR4/TNFRSF10D Monoclonal Antibody (Catalog # MAB633R).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human TRAIL R4/TNFRSF10D DuoSet ELISA Kit (Catalog # DY633) for convenient development of a sandwich ELISA.
Flow Cytometry
2.5 µg/106 cells
Sample: Human whole blood granulocytes
Sample: Human whole blood granulocytes
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (occipital cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human brain (occipital cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
0.1 µg/mL
Sample: Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR)
Sample: Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR)
Neutralization
Measured by its ability to neutralize TRAIL R4/TNFRSF10D-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.03-0.1 µg/mL in the presence of 10 ng/mL Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAILR4/TNFRSF10D
Human TRAIL R4, also called decoy receptor 2 (DcR2) and TRUNND (TRAIL receptor with a truncated death domain), is a type 1, TNF R family membrane protein, which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R4 is referred to as TNFRSF10D. TRAIL R4 is unique among the TRAIL receptors in that its cytoplasmic domain contains a truncated consensus death domain motif. Binding of TRAIL R4 does not result in an apoptotic signal. Overexpression of TRAIL R4 can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. The human soluble TRAIL R4/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.
References
- Griffith, T.S. et al. (1998) Curr. Opin. Immunol. 10:559.
- Pan, G. et al. (1998) FEBS lett 424:41.
- Marsters, S.A. et al. (1997) Cur. Biol. 7:1003.
- Degli-Esposti, M.A. et al. (1997) Immunity 7:813.
Long Name
TRAIL Receptor 4
Alternate Names
CD264, DcR2, TNFRSF10D, TRAIL R4
Entrez Gene IDs
8793 (Human)
Gene Symbol
TNFRSF10D
UniProt
Additional TRAILR4/TNFRSF10D Products
Product Documents for Human TRAILR4/TNFRSF10D Antibody
Product Specific Notices for Human TRAILR4/TNFRSF10D Antibody
For research use only
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