Human TRAILR4/TNFRSF10D Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF633
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair), Western Blot
Cited:
Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL R4/TNFRSF10D
Ala56-His211
Accession # Q9UBN6
Ala56-His211
Accession # Q9UBN6
Specificity
Detects human TRAIL R4/TNFRSF10D in ELISAs and Western blots. In sandwich immunoassays, less than 3.5% cross-reactivity with recombinant human (rh) TRAIL R3 is observed and less than 0.5% cross-reactivity with rhTRAIL R1, rhTRAIL R2, rhTRAIL, rhTNF-alpha, and rhTNF-beta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human TRAILR4/TNFRSF10D Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR)
Sample: Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR)
Human TRAIL R4/TNFRSF10D Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAILR4/TNFRSF10D
Human TRAIL R4, also called decoy receptor 2 (DcR2) and TRUNND (TRAIL receptor with a truncated death domain), is a type 1, TNF R family membrane protein, which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R4 is referred to as TNFRSF10D. TRAIL R4 is unique among the TRAIL receptors in that its cytoplasmic domain contains a truncated consensus death domain motif. Binding of TRAIL R4 does not result in an apoptotic signal. Overexpression of TRAIL R4 can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. The human soluble TRAIL R4/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.
References
- Griffith, T.S. et al. (1998) Curr. Opin. Immunol. 10:559.
- Pan, G. et al. (1998) FEBS lett 424:41.
- Marsters, S.A. et al. (1997) Cur. Biol. 7:1003.
- Degli-Esposti, M.A. et al. (1997) Immunity 7:813.
Long Name
TRAIL Receptor 4
Alternate Names
CD264, DcR2, TNFRSF10D, TRAIL R4
Entrez Gene IDs
8793 (Human)
Gene Symbol
TNFRSF10D
UniProt
Additional TRAILR4/TNFRSF10D Products
Product Documents for Human TRAILR4/TNFRSF10D Biotinylated Antibody
Product Specific Notices for Human TRAILR4/TNFRSF10D Biotinylated Antibody
For research use only
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