Human TRIM21 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6219
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TRIM21
Met1-Tyr475
Accession # P19474
Met1-Tyr475
Accession # P19474
Specificity
Detects human TRIM21 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human TRIM21 Antibody
Detection of Human TRIM21 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human kidney tissue. PVDF Membrane was probed with 0.5 µg/mL of Human TRIM21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6219) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for TRIM21 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
TRIM21 in Human PBMCs.
TRIM21 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated for 8 hours with 20 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) using Human TRIM21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of TRIM21 in HUVEC Human Umbilical Vein Endothelial Cells and A549 Human Lung Carcinoma Cells (Both Positive Controls).
TRIM21 was detected in immersion fixed HUVEC Human Umbilical Vein Endothelial Cells and A549 Human Lung Carcinoma Cells (Both Positive Controls) using Sheep Anti-Human TRIM21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6219) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human TRIM21 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with Recombinant Human IFN-gamma (Catalog # 285-IF) and immersion fixed HUVEC Human Umbilical Vein Endothelial Cells and A549 Human Lung Carcinoma Cells (Both Positive Controls)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with Recombinant Human IFN-gamma (Catalog # 285-IF) and immersion fixed HUVEC Human Umbilical Vein Endothelial Cells and A549 Human Lung Carcinoma Cells (Both Positive Controls)
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and human kidney tissue
Sample: HeLa human cervical epithelial carcinoma cell line and human kidney tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRIM21
TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52 kDa Ro Protein/Ro52, and RING finger protein 81) is a 52-56 kDa member of the RING finger B box coiled coil family of proteins. It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules. TRIM21 ubiquitinates select proteins. In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production. In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity. Human TRIM21 is 475 amino acids (aa) in length and contains one E3 ligase RING finger domain (aa 16-55), a B Box type zinc finger region (aa 92-123), a coiled coil region (aa 128-238) and a C-terminal SPRY/B30.2 Ig binding domain (aa 268-465). TRIM21 is reported to form trimers. Over aa 195‑293, human TRIM21 exhibits 72% aa identity with mouse TRIM21.
Long Name
Tripartite Motif-containing Protein 21
Alternate Names
RNF81, Ro52, SSA1
Gene Symbol
TRIM21
UniProt
Additional TRIM21 Products
Product Documents for Human TRIM21 Antibody
Product Specific Notices for Human TRIM21 Antibody
For research use only
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