Human Tryptase alpha/beta-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3796
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 349406
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tryptase alpha/beta-1
Ile31-Pro275
Accession # AAD13876
Ile31-Pro275
Accession # AAD13876
Specificity
Detects human Tryptase alpha/beta-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50‑100% cross-reactivity with recombinant human (rh) TPSAB1 and recombinant mouse (rm) Mcpt6 is observed, 20‑100% cross-reactivity with rmMcpt7 is observed, and no cross-reactivity with rhTPSG1, rmTPS5, rmMcpt1, or rmMcpt11 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Tryptase alpha/beta-1 Antibody
Detection of Tryptase alpha/beta-1 in Human Lung.
Tryptase alpha/beta-1 was detected in immersion fixed paraffin-embedded sections of Human Lung using Mouse Anti-Human Tryptase alpha/beta-1 Monoclonal Antibody (Catalog # MAB3796) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to mast cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Tryptase alpha/beta-1 Antibody
Application
Recommended Usage
Immunohistochemistry
0.1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Lung
Sample: Immersion fixed paraffin-embedded sections of Human Lung
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796‑SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796‑SE), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796-SE)
Sample: Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796-SE)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tryptase alpha/beta-1
Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). They are stored in secretory granules of mast cells, where they form active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, beta tryptases are resistant to macromolecule protease inhibitors (2). When mast cells are activated, beta tryptases are released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). beta tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic disorders.
References
- Caughey, G. H. 2004, in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1535.
- Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
- Hallgren, J. and G. Pejler (2006) FEBS J. 273:1871.
Alternate Names
TPS2, TPSB2, Tryptase beta-2, Tryptase beta2, Tryptase-C
Gene Symbol
TPSB2
UniProt
Additional Tryptase alpha/beta-1 Products
Product Documents for Human Tryptase alpha/beta-1 Antibody
Product Specific Notices for Human Tryptase alpha/beta-1 Antibody
For research use only
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