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Human TSHR Alexa Fluor® 405-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB65342V

R&D Systems, part of Bio-Techne
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FAB65342V-100UG

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 484404

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TSHR
Accession # P16473

Specificity

Detects human TSHR in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human TSHR Alexa Fluor® 405-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human TSH R and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitution default not known

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TSHR

Thyroid stimulating hormone receptor (TSH R; also thyrotropin receptor) is an 85 kDa (unglycosylated) member of the G-protein coupled receptor 1 family. Human TSH R is synthesized as a 764 amino acid (aa) precursor that contains a 20 aa signal sequence, a 393 aa extracellular domain (ECD), a 269 aa membrane spanning domain consisting of seven transmembrane segments, and an 82 aa cytoplasmic domain. The ECD contains six leucine-rich repeats (LRR) and six potential sites of N-linked glycosylation. There are two isoforms, long and short, produced by a splicing variant corresponding to aa 253-764 in the long isoform that is missing in the short isoform. Human TSH R is 86-87% aa identical to mouse and rat TSH R. TSH R is the receptor for thyroid stimulating hormone, which plays a central role in controlling thyroid cell metabolism.

Long Name

Thyroid Stimulating Hormone Receptor

Alternate Names

CHNG1, LGR3, TSH R

Entrez Gene IDs

7253 (Human); 22095 (Mouse)

Gene Symbol

TSHR

UniProt

Additional TSHR Products

Product Documents for Human TSHR Alexa Fluor® 405-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TSHR Alexa Fluor® 405-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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