HEK293 human embryonic kidney cell line transfected with either (A) human TSH R or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB65342) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TSHR Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human TSH R and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSHR

Thyroid stimulating hormone receptor (TSH R; also thyrotropin receptor) is an 85 kDa (unglycosylated) member of the G-protein coupled receptor 1 family. Human TSH R is synthesized as a 764 amino acid (aa) precursor that contains a 20 aa signal sequence, a 393 aa extracellular domain (ECD), a 269 aa membrane spanning domain consisting of seven transmembrane segments, and an 82 aa cytoplasmic domain. The ECD contains six leucine-rich repeats (LRR) and six potential sites of N-linked glycosylation. There are two isoforms, long and short, produced by a splicing variant corresponding to aa 253-764 in the long isoform that is missing in the short isoform. Human TSH R is 86-87% aa identical to mouse and rat TSH R. TSH R is the receptor for thyroid stimulating hormone, which plays a central role in controlling thyroid cell metabolism.

Long Name

Thyroid Stimulating Hormone Receptor

Alternate Names

CHNG1, LGR3, TSH R

Entrez Gene IDs

7253 (Human); 22095 (Mouse)

Gene Symbol

TSHR

UniProt

P16473

Additional TSHR Products

Product Documents for Human TSHR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TSHR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TSHR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB65342

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TSHR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TSHR Antibody

Detection of TSH R in HEK293 Human Cell Line Transfected with Human TSH R and eGFP by Flow Cytometry.