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Human Ubiquitin Antibody

R&D Systems, part of Bio-Techne | Catalog # A-100

R&D Systems, part of Bio-Techne
Discontinued Product
A-100 has been discontinued. An alternative/replacement product is available: A-104. View all Ubiquitin products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Plant

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Product Specifications

Immunogen

Purified human recombinant ubiquitin

Specificity

The antibody cross-reacts with mouse, rat and human ubiquitin; but not with Nedd8 or Sumo1 proteins. This reagent is non-functional for immunoprecipitation.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Human Ubiquitin Antibody

Application
Recommended Usage

Western Blot

Recommended dilution range for Western blot analysis is 1:500 to 1:1500. At a dilution of 1:1000, 10 ng of monomeric ubiquitin can be detected.

Reviewed Applications

Read 1 review rated 4 using A-100 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a solution in PBS.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Ubiquitin

Rabbit polyclonal antibody with a high specificity and affinity for ubiquitin.  The antibody cross-reacts with free ubiquitin, mono-ubiquitin and poly-ubiquitin chain conjugates.

References

    1. Haas, A.L. and Bright, P.M. (1985) J. Biol. Chem 260:12464.
    2. Guarino, L.A. et al. (1995) Cell  80:301-309.
    3. Takada, K. et al. (1995) Eur. J. Biochem.   233:42.

    Alternate Names

    UBB

    Entrez Gene IDs

    7314 (Human); 298693 (Rat)

    Gene Symbol

    UBB

    Additional Ubiquitin Products

    Product Documents for Human Ubiquitin Antibody

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Product Specific Notices for Human Ubiquitin Antibody

    For research use only

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