Human Use1/UBE2Z Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8154
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Use1/UBE2Z
Met1-Val354
Accession # Q9H832
Met1-Val354
Accession # Q9H832
Specificity
Detects human USE1/UBE2Z in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Use1/UBE2Z Antibody
Detection of Human Use1/UBE2Z by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and PANC-1 human pancreatic carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Use1/UBE2Z Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8154) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Use1/UBE2Z at approximately 39-41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Use1/UBE2Z Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: A549 human lung carcinoma cell line and PANC‑1 human pancreatic carcinoma cell line
Sample: A549 human lung carcinoma cell line and PANC‑1 human pancreatic carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Use1/UBE2Z
Uba6-specific E2 Conjugating Enzyme 1 (Use1), also known as Ubiquitin-conjugating Enzyme E2Z (UBE2Z), is a 38 to 43 kDa member of the Ubiquitin-conjugating (E2) enzyme family that is highly expressed in human placenta, pancreas, spleen, and testis. Use1 has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin. It functions specifically with the UBE1L2/UBA6 Ubiquitin-activating (E1) enzyme, rather than the UBE1 E1 enzyme, for Ubiquitin conjugation. Expression is enriched in the cytoplasm, where it mediates the ubiquitination of RGS4 and RGS5 via the N-end rule proteolytic pathway. Use1 also functions with UBE1L2/UBA6 to mediate the conjugation of HLA F-associated Transcript 10 (FAT10), a Ubiquitin-like protein, and is auto-FAT10ylated in response to TNF-alpha and IFN-gamma. Full-length human Use1 is 354 amino acids (aa) and shares 100% sequence identity with mouse and rat Use1. A potential smaller isoform lacks aa 1-108.
Additional Use1/UBE2Z Products
Product Documents for Human Use1/UBE2Z Antibody
Product Specific Notices for Human Use1/UBE2Z Antibody
For research use only
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