Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/mL Recombinant Human TNF-alpha (210-TA) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VCAM-1/CD106 Monoclonal Antibody (Catalog # MAB8091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for VCAM-1/CD106 at approximately 110 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of VCAM‑1/CD106 in HuT 78 Human Cell Line by Flow Cytometry.

HuT 78 human cutaneous T cell lymphoma cell line was stained with Mouse Anti-Human VCAM-1/CD106 Monoclonal Antibody (Catalog # MAB8091, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

VCAM-1/CD106 in Human Spleen.

VCAM-1/CD106 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human VCAM-1/CD106 Monoclonal Antibody (Catalog # MAB8091) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Applications for Human VCAM-1/CD106 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HuT 78 human cutaneous T cell lymphoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human splee

Simple Western

20 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

2 µg/mL
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha, Catalog # 210-TA

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB8091 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

VCAM-1, also known as CD106, is an immunoglobulin (Ig)-like adhesion molecule that is mainly expressed in endothelial cells and other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes (1, 2). It plays a critical role in inflammation by recruiting leukocytes to acute and chronic inflammation sites (3, 4). Alternatively-spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 aa extracellular domain (ECD) that includes seven C2-type immunoglobulin domains, a 22 aa transmembrane segment, and a 19 amino acid (aa) cytoplasmic tail. Within the ECD, human VCAM-1 shares 75% and 76% aa sequence identity with the mouse and rat VCAM-1, respectively. VCAM-1 binds to leukocyte integrins alpha 4 beta 1 (VLA-4) and alpha 4 beta 7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. The VCAM-1:VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation (5). ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals, but elevated levels of serum VCAM-1 are indicative of future Atrial Fibrillation incident as well as liver disease (6, 7). Tumor cells use overexpression of VCAM-1 as means of escaping immune surveillance (8).

References

Vonderheide, R.H. et al. (1994) J. Cell Biol. 125:215.
Cybulsky, M.I. et al. (1991) Proc. Natl. Acad. Sci. USA 88:7859.
Luster, A.D. et al. (2005) Nat. Immunol. 6:1182.
Osborn, L. et al. (1989) Cell 59:1203
Langer. H.F. et al. 2009. J Cell Mol Med. 13:1211.
Willeit.K. et al. 2017. JAMA Cardiol. 2:516.
Lo Iacono.O. et al. 2008. Liver Int. 28:1129.
Wu.T.C. et al. 2007. Cancer Research. 67:6003

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Gene Symbol

VCAM1

UniProt

P19320-1

Additional VCAM-1/CD106 Products

Product Documents for Human VCAM-1/CD106 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human VCAM-1/CD106 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human VCAM-1/CD106 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8091

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

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Product Summary for Human VCAM-1/CD106 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human VCAM-1/CD106 Antibody

Detection of Human VCAM-1/CD106 by Western Blot.