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Human VCAM-1/CD106 Antibody

R&D Systems, part of Bio-Techne | Catalog # BBA5

R&D Systems, part of Bio-Techne
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BBA5

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Adhesion Blockade, Immunocytochemistry, Immunoprecipitation, Western Blot

Cited:

Blocking, ELISA Development, ELISA Development (Capture), Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BBIG-V1

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human VCAM-1/CD106 Antibody

VCAM-1/CD106 antibody in HUVEC Cells by Immunocytochemistry (ICC).

VCAM‑1/CD106 in HUVEC Cells.

VCAM-1/CD106 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human VCAM-1/CD106 Monoclonal Antibody (Catalog # BBA5) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.This antibody is not suitable for labeling formalin-fixed, paraffin-embedded tissue./P>

VCAM-1/CD106 in HUVEC cells treated with TNF alpha (positive) and untreated HUVEC cells (negative).

VCAM-1/CD106 was detected in immersion fixed HUVEC cells treated with TNF alpha (positive) and untreated HUVEC cells (negative) using Mouse Anti-Human VCAM-1/CD106 Monoclonal Antibody (Catalog # BBA5) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human VCAM-1/CD106 Antibody

Application
Recommended Usage

Adhesion Blockade

The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human VCAM-1/CD106 (Catalog # ADP5, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 30 µg/mL of the antibody.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells, and immersion fixed HUVEC human umbilical vein endothelial cells activated with recombinant human TNF-alpha (Catalog # 210-TA). This antibody is not suitable for labeling formalin-fixed, paraffin-embedded tissue.

Immunoprecipitation

10 µg/mL
Sample: Radiolabelled activated HUVEC human umbilical vein endothelial cells, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human VCAM‑1/CD106 (Catalog # 809-VR) under non-reducing conditions only

Reviewed Applications

Read 1 review rated 4 using BBA5 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha4 beta7 to promote leukocyte adhesion at sites of inflamed vasculature.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for Human VCAM-1/CD106 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VCAM-1/CD106 Antibody

For research use only

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