Human VHL Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB114352

R&D Systems, part of Bio-Techne
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MAB114352-100
MAB114352-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1066408

View all VHL Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VHL
Asn150-Asp213
Accession # P40337

Specificity

Detects human VHL in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human VHL Antibody

Detection of VHL in Daudi Human Cell Line.

VHL was detected in formalin fixed Daudi human Burkitt's lymphoma cell line using Mouse Anti-Human VHL Monoclonal Antibody (Catalog # MAB114352) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human VHL Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Formalin fixed Daudi human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VHL

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues.  Targets of VHL include HIF1 alpha, beta2 adrenergic receptor, ZHX2 and others.  In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families.  ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.  

References

  1. Kamura, T. et al. (1998) Genes & Dev. doi: 10.1101/gad.12.24.3872.
  2. Okumura, F. et al. (2012) Front. Oncol. doi: 10.3389/fonc.2012.00010.
  3. Xie, L. et al. (2009) Sci. Signaling doi: 10.1126/scisignal.2000444.
  4. Zhang, J. et al. (2018) Science doi: 10.1126/science.aap8411.

Long Name

Von Hippel-Lindau Tumor Suppressor

Alternate Names

Protein G7, pVHL

Entrez Gene IDs

7428 (Human); 22346 (Mouse)

Gene Symbol

VHL

UniProt

P40337 (Human)

Additional VHL Products

Product Documents for Human VHL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VHL Antibody

For research use only

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