Human VSIG1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB48182
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 986709
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VSIG1
Val22-Gly234
Accession # Q86XK7
Val22-Gly234
Accession # Q86XK7
Specificity
Detects human VSIG1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human VSIG1 Antibody
Detection of Human VSIG1 by Western Blot.
Western blot shows lysates of human stomach tissue and human testis tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human VSIG1 Monoclonal Antibody (Catalog # MAB48182) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VSIG1 at approximately 50-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VSIG1 in Human Testis.
VSIG1 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human VSIG1 Monoclonal Antibody (Catalog # MAB48182) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human VSIG1 by Simple WesternTM.
Simple Western lane view shows lysates of human stomach, loaded at 0.2 mg/mL. A specific band was detected for VSIG1 at approximately 56 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human VSIG1 Monoclonal Antibody (Catalog # MAB48182). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human VSIG1 Antibody
Application
Recommended Usage
Immunohistochemistry
0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis
Sample: Immersion fixed paraffin-embedded sections of human testis
Simple Western
20 µg/mL
Sample: Human stomach
Sample: Human stomach
Western Blot
2 µg/mL
Sample: Human stomach tissue and Human testis tissue
Sample: Human stomach tissue and Human testis tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VSIG1
VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein A34) is a variably glycosylated 55‑70 kDa member of the JAM family of proteins. It has restricted expression, being limited to testicular germ cells plus pancreatic duct and gastric epithelium. VSIG1 is likely to serve as an adhesion molecule. Mature human VSIG1 is 366 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 211 aa extracellular domain (ECD). The ECD contains one V-type (aa 22‑132) and one C2-type Ig-like domain (aa 140‑227). Over aa 22‑234, human VSIG1 is 83% aa identical to both mouse and canine VSIG1. At least one potential splice variant exists in human. It shows an insertion of 36 aa after Ser72 and a deletion of aa 133‑387.
Long Name
V-Set and Immunoglobulin Domain Containing 1
Alternate Names
GPA34
Gene Symbol
VSIG1
UniProt
Additional VSIG1 Products
Product Documents for Human VSIG1 Antibody
Product Specific Notices for Human VSIG1 Antibody
For research use only
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