Human VSIG1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4818
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VSIG1
Val22-Gly234
Accession # Q86XK7
Val22-Gly234
Accession # Q86XK7
Specificity
Detects human VSIG1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human (rh) VSIG2, rhVSIG3, rhVSIG4, and recombinant mouse VSIG1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human VSIG1 Antibody
Detection of Human VSIG1 by Western Blot.
Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human VSIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4818) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VSIG1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VSIG1 in Human Testis.
VSIG1 was detected in immersion fixed frozen sections of human testis using 10 µg/mL Sheep Anti-Human VSIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4818) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Human VSIG1 by Simple WesternTM.
Simple Western lane view shows lysates of human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for VSIG1 at approximately 115 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human VSIG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4818) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human VSIG1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of human testis
Sample: Immersion fixed frozen sections of human testis
Simple Western
10 µg/mL
Sample: Human testis tissue
Sample: Human testis tissue
Western Blot
0.2 µg/mL
Sample: Human testis tissue
Sample: Human testis tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VSIG1
VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein A34) is a variably glycosylated 55‑70 kDa member of the JAM family of proteins. It has restricted expression, being limited to testicular germ cells plus pancreatic duct and gastric epithelium. VSIG1 is likely to serve as an adhesion molecule. Mature human VSIG1 is 366 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 211 aa extracellular domain (ECD). The ECD contains one V-type (aa 22‑132) and one C2-type Ig-like domain (aa 140‑227). Over aa 22‑234, human VSIG1 is 83% aa identical to both mouse and canine VSIG1. At least one potential splice variant exists in human. It shows an insertion of 36 aa after Ser72 and a deletion of aa 133‑387.
Long Name
V-Set and Immunoglobulin Domain Containing 1
Alternate Names
GPA34
Gene Symbol
VSIG1
UniProt
Additional VSIG1 Products
Product Documents for Human VSIG1 Antibody
Product Specific Notices for Human VSIG1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...