Human WASP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3070
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 336925
Product Specifications
Immunogen
E. coli-derived recombinant human WASP
Lys230-Gln310
Accession # P42768
Lys230-Gln310
Accession # P42768
Specificity
Detects human WASP in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human WASP Antibody
WASP in U937 Human Cell Line.
WASP was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human WASP Monoclonal Antibody (Catalog # MAB3070) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human WASP Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Western Blot
1 µg/mL
Sample: Recombinant Human WASP
Sample: Recombinant Human WASP
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB3070 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WASP
Long Name
Wiskott-Aldrich Syndrome Protein
Alternate Names
Thrombocytopenia 1, WAS
Gene Symbol
WAS
UniProt
Additional WASP Products
Product Documents for Human WASP Antibody
Product Specific Notices for Human WASP Antibody
For research use only
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