Human ZEB1 Antibody

ZEB1 in MDA‑MB‑231 Human Cell Line.

ZEB1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB6708) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of ZEB-1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry

MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human ZEB1 Monoclonal Antibody (Catalog # MAB6708, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Human Human ZEB1 Antibody by Western Blot

RAB25 is a ZEB1 target gene. (A) RAB25 expression positively correlated with E-cadherin but negatively with ZEB1 in a series of lung cancer cell lines. RNA expression was measured by quantitative real-time PCR in 22 NSCLC cell lines, two NHBE cultures, and two immortalized human airway primary cell lines (BAES2B and FC6625-2 3KT). Cells are ranked from left to right with increasing RAB25 mRNA level. Values are expressed as percent of the geometric mean between GAPDH and actin mRNA. The experiment was done twice with qRT-PCR in duplicate. (B) RAB25 mRNA level is decreased by ZEB1 overexpression in H358 FlipIn ZEB1 cells (left) and by TGF beta treatment in H358 EV control cells (right). Values are expressed as percent of GAPDH for three independent experiments with qRT-PCR in duplicate. Bars = SD. (C) Western blot: RAB25 protein level is decreased by ZEB1 overexpression during 1 to 5 days of DOX treatment in H358 FlipIn ZEB1cells. E-cadherin is decreased as well. Actin is the loading control. Protein molecular weights are indicated in kDa on the left. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24216980), licensed under a CC-BY license. Not internally tested by R&D Systems.