Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Block/Neutralize, CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Inhibition Activity, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG Clone # 3E2B

Format

Azide and BSA Free

Concentration

1 mg/ml

View all ICAM-1/CD54 Antibodies »

Product Specifications

Immunogen

Mouse ICAM-1 (CD54)

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Scientific Data Images for ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free

Western Blot: ICAM-1/CD54 Antibody (3E2B)Azide and BSA Free [NBP2-22540]

Western Blot: ICAM-1/CD54 Antibody (3E2B)Azide and BSA Free [NBP2-22540]

Western Blot: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Analysis of 25 ug of NIH-3T3 (lane 1), mouse spleen (lane 2) and Ramos (lane 3) cell lysates.
Immunocytochemistry/ Immunofluorescence: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunocytochemistry/ Immunofluorescence: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Analysis of CD54 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free [NBP2-22540]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (3E2B) [NBP2-22540] - Normal biopsies of deparaffinized Mouse spleen tissue.
View 3 more images

Applications for ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:10 - 1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ICAM-1/CD54

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Entrez Gene IDs

15894 (Mouse)

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ICAM-1/CD54 Antibody (3E2B) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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