ICAM-1/CD54 Antibody (MEM-111) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-318

Clone MEM-111 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB500-318-25ug
NB500-318
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat, Bovine

Cited:

Human, Rat, Bovine

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-111

Format

BSA Free

Concentration

1 mg/ml

View all ICAM-1/CD54 Antibodies »

Product Specifications

Immunogen

Raji human Burkitt's lymphoma cell line (NM_000201.2).

Specificity

The antibody MEM-111 reacts with an extracellular epitope CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. HLDA VI; WS Code AS A049

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ICAM-1/CD54 Antibody (MEM-111) - BSA Free

Western Blot: ICAM-1/CD54 Antibody (MEM-111)BSA Free [NB500-318]

Western Blot: ICAM-1/CD54 Antibody (MEM-111)BSA Free [NB500-318]

Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - ICAM-1 protein expression levels in rat lung tissue after the different treatments in each group. Immunoblotting showed increase in ICAM-1 after one course of paclitaxel compared with normal lung tissue; p < 0.01. These changes were reversed by using parecoxib in combination with paclitaxel, while the group that received parecoxib treatment alone resembled the Con group. The bar graph under the protein strap represents the corresponding band intensities; the data are presented as x +/- s and corrected by GA. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep12977), licensed under a CC-BY license.
Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) - BSA Free [NB500-318]

Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) - BSA Free [NB500-318]

Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE
Western Blot: ICAM-1/CD54 Antibody (MEM-111)BSA Free [NB500-318]

Western Blot: ICAM-1/CD54 Antibody (MEM-111)BSA Free [NB500-318]

Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Of CD54 expression in activated (A) and nonactivated (B) Huvec cells by antibody MEM-111. Lower bands represent tubulin as a loading control.
View 2 more images

Applications for ICAM-1/CD54 Antibody (MEM-111) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Flow Cytometry

0.5-4 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
WB: Use under non-reducing conditions. Use in IHC-Frozen reported in scientific literature (PMID 26256764). This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ICAM-1/CD54

CD54 (ICAM1) is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase in inflammation. Also, ICAM1 is detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (MEM-111) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ICAM-1/CD54 Antibody (MEM-111) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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