ICAM-1/CD54 Antibody (ST0487)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67518

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ST0487

Concentration

1 mg/ml

View all ICAM-1/CD54 Antibodies »

Product Summary for ICAM-1/CD54 Antibody (ST0487)

Immunogen

Synthetic peptide within human ICAM-1/CD54 aa 30-70. (SwissProt: P05362 Human)

Localization

Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ICAM-1/CD54 Antibody (ST0487)

Western Blot: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Western Blot: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Western Blot: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of ICAM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: HUVEC cell lysate
Immunocytochemistry/ Immunofluorescence: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Immunocytochemistry/ Immunofluorescence: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Staining ICAM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.
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Applications for ICAM-1/CD54 Antibody (ST0487)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ICAM-1/CD54

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (ST0487)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ICAM-1/CD54 Antibody (ST0487)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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