Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Neutralization

Label

Janelia Fluor 549

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 25723

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IFN-gamma Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IFN-gamma
Gln24-Gln166
Accession # AAP20098.1

Specificity

Detects human IFN-gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma, recombinant rat IFN-gamma, or recombinant porcine IFN-gamma is observed. Neutralizes the bioactivity of recombinant human IFN-gamma and will not neutralize the activity of rat or mouse IFN-gamma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for IFN-gamma Antibody (25723) [Janelia Fluor® 549]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IFN-gamma

IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN-gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN-gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Gene Symbol

IFNG

Additional IFN-gamma Products

Product Documents for IFN-gamma Antibody (25723) [Janelia Fluor® 549]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IFN-gamma Antibody (25723) [Janelia Fluor® 549]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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