IFN-gamma Antibody (43-11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31044

Novus Biologicals, part of Bio-Techne
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NBP2-31044
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Key Product Details

Species Reactivity

Human

Applications

Block/Neutralize, Chemiluminescence Immunoassay, Cytokine immunotrapping Assay, ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 43-11

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IFN-gamma Antibodies »

Product Specifications

Immunogen

Monoclonal Antibody to Human IFN-g (ELISA Capture)-Azide Free

Specificity

This antibody recognizes both the recombinant and the natural form of human interferon-gamma (IFN-gamma) (1). This antibody does not react with human IL-4, IFN-alpha or acid inactivated IFN-gamma , and it shows only a minimal reactivity with heatinactivated IFN-g (

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IFN-gamma Antibody (43-11) - BSA Free

Western Blot: IFN-gamma Antibody (43-11) [NBP2-31044]

Western Blot: IFN-gamma Antibody (43-11) [NBP2-31044]

Western Blot: IFN-gamma Antibody (43-11) [NBP2-31044] - analysis of IFN-gamma in HEK293 cell lysate using anti-IFN-gamma antibody. Image from verified customer review.

Applications for IFN-gamma Antibody (43-11) - BSA Free

Application
Recommended Usage

Chemiluminescence Immunoassay

>0.2 pg/ml

ELISA

>15 pg/ml
Application Notes
WB reported in a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-31044 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 8.0)

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFN-gamma

ELISA: Purified anti-human IFN-g antibody (clone 43-11) can be used as the capture reagent in a two-site sandwich ELISA for measuring human IFN-g levels in conjunction with the biotinylated form of the anti-human IFN-g monoclonal (clone 45-15 Cat. No. IMG-211B) as the detecting antibody (1), and recombinant human IFN-g as the standard. The optimal concentration of clone 43-11 for coating ELISA plates was 5 mg/ml. This ELISA measured human IFN-g with a detection limit of 37 pg/ml (1). The detection limit can be increased to 15 pg/ml with the use of a bead-ELISA assay (1). Chemiluminescent Immuno Assay (CLIA): Clones 43-11 and 45-15 (Cat. No. IMG-210 and 211) can be used together as an antibody pair to implement a highly sensitive Chemiluminescent Immuno Assay (CLIA) for human IFN-g (0.5 pg/ml as the detection limit) and a bead-based CLIA (detection limit of 0.2 pg/ml) (1). Cytokine Immunotrapping Assay (CITA): Furthermore clones 43-11 and 45-15 (Cat. No. IMG-210 and 211) can be used in combination to study the early production of human IFN-g in a highly sensitive chemiluminescent based Cytokine Immuno-trapping Assay (CITA) (2). Neutralization: This antibody is not suitable for neutralization studies. For neutralization of the biologic activity of human IFN-g the use of clone 45-15 (Cat. No. IMG-211E) is recommended (1).

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Gene Symbol

IFNG

UniProt

NP_004855 (Human)

Additional IFN-gamma Products

Product Documents for IFN-gamma Antibody (43-11) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IFN-gamma Antibody (43-11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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