IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81044
Recombinant Monoclonal Antibody
![Western Blot: IFN-gamma Antibody (D9D10)ChimericAzide and BSA Free [NBP2-81044]](https://resources.bio-techne.com/images/products/IFN-gamma-Antibody-D9D10-Western-Blot-NBP2-81044-img0001.jpg "Western Blot: IFN-gamma Antibody (D9D10)ChimericAzide and BSA Free [NBP2-81044]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Block/Neutralize, ELISA, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # D9D10
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
FPLC-purified natural human IFN-gamma. Refer to the original article
Reactivity Notes
Cross Reactivity: Baboon
Specificity
Binds human IFN-gamma, a dimerized soluble cytokine that is the only member of the type II class of interferons.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free
Western Blot: IFN-gamma Antibody (D9D10)ChimericAzide and BSA Free [NBP2-81044]
Western Blot: IFN-gamma Antibody (D9D10) [NBP2-81044] - Western Blot using anti-IFN gamma antibody (D9D10) [NBP2-81044]. Human thymus lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of D9D10 [NBP2-81044] at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for IFN gamma is 19.3kDa, though the form shown here is likely glycosylated. NBP2-81044 successfully detected IFN gamma in human thymus samples.Applications for IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody can be used to detect both native and recombinant intereferon gamma (IFNg). It is reported only to bind to the functional, dimeric form of IFNg.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: IFN-gamma
Long Name
Interferon gamma
Alternate Names
IFG, IFI, IFNG, IFNgamma
Gene Symbol
IFNG
Additional IFN-gamma Products
Product Documents for IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free
Product Specific Notices for IFN-gamma Antibody (D9D10) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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