IGF-I/IGF-1 Antibody (IGF1/1020)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44968

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # IGF1/1020

Concentration

0.2 mg/ml

View all IGF-I/IGF-1 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human IGF-I/IGF-1 protein (Uniprot: P05019)

Localization

Cytoplasmic (Secreted)

Specificity

This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-48001)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for IGF-I/IGF-1 Antibody (IGF1/1020)

Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) [NBP2-44968]

Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) [NBP2-44968]

Immunocytochemistry/Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) [NBP2-44968] - Immunofluorescence Analysis of HeLa cells labeling IGF-1 with IGF-I/IGF-1 Antibody (IGF1/1020) labeled with CF647R (Green). Nuclei are counter stained with DAPI (Blue).

Applications for IGF-I/IGF-1 Antibody (IGF1/1020)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-4 ug/ml
Application Notes
In-vitro Neutralization of Biological Activity of IGF-I/IGF-1: Order Ab without Azide.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IGF-I/IGF-1

Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Gene Symbol

IGF1

UniProt

P05019

Additional IGF-I/IGF-1 Products

Product Documents for IGF-I/IGF-1 Antibody (IGF1/1020)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IGF-I/IGF-1 Antibody (IGF1/1020)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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