IGF-I/IGF-1 Antibody (IGF1/1020)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44968
![Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) [NBP2-44968]](https://resources.bio-techne.com/images/products/IGF-I-IGF-1-Antibody-IGF1-1020-Immunocytochemistry-Immunofluorescence-NBP2-44968-img0001.jpg "Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) [NBP2-44968]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Rabbit
Cited:
Rat
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # IGF1/1020
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human IGF-I/IGF-1 protein (Uniprot: P05019)
Localization
Cytoplasmic (Secreted)
Specificity
This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-48001)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for IGF-I/IGF-1 Antibody (IGF1/1020)
Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) [NBP2-44968]
Immunocytochemistry/Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) [NBP2-44968] - Immunofluorescence Analysis of HeLa cells labeling IGF-1 with IGF-I/IGF-1 Antibody (IGF1/1020) labeled with CF647R (Green). Nuclei are counter stained with DAPI (Blue).Applications for IGF-I/IGF-1 Antibody (IGF1/1020)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-4 ug/ml
Application Notes
In-vitro Neutralization of Biological Activity of IGF-I/IGF-1: Order Ab without Azide.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: IGF-I/IGF-1
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Alternate Names
IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C
Gene Symbol
IGF1
UniProt
Additional IGF-I/IGF-1 Products
Product Documents for IGF-I/IGF-1 Antibody (IGF1/1020)
Product Specific Notices for IGF-I/IGF-1 Antibody (IGF1/1020)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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