IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48001
![Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free [NBP2-48001]](https://resources.bio-techne.com/images/products/IGF-I-IGF-1-Antibody-IGF1-1020-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-48001-img0001.jpg "Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free [NBP2-48001]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Rabbit
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # IGF1/1020
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free
Immunogen
Recombinant full-length human IGF-I/IGF-1 protein (Uniprot: P05019)
Localization
Cytoplasmic (Secreted)
Specificity
This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44968).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free [NBP2-48001]
Immunocytochemistry/Immunofluorescence: IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free [NBP2-48001] - Immunofluorescence Analysis of HeLa cells labeling IGF-1 with IGF-I/IGF-1 Antibody (IGF1/1020) labeled with CF647R (Green). Nuclei are counter stained with DAPI (Blue).Applications for IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunofluorescence
1 - 2 ug/ml
Application Notes
In-vitro Neutralization of Biological Activity of IGF-I/IGF-1: Order Ab without Azide.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: IGF-I/IGF-1
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Alternate Names
IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C
Gene Symbol
IGF1
Additional IGF-I/IGF-1 Products
Product Documents for IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free
Product Specific Notices for IGF-I/IGF-1 Antibody (IGF1/1020) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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