IGF-I R/IGF1R Antibody (3G5C1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-87052

Novus Biologicals, part of Bio-Techne
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NB110-87052
NB110-87052SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3G5C1

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all IGF-I R/IGF1R Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of IGF-I R/IGF1R (AA: 1101-1367) expressed in E. Coli.

Localization

Membrane; Single-pass type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IGF-I R/IGF1R Antibody (3G5C1) - BSA Free

Western Blot: IGF-I R/IGF1R Antibody (3G5C1)BSA Free [NB110-87052]

Western Blot: IGF-I R/IGF1R Antibody (3G5C1)BSA Free [NB110-87052]

Western Blot: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis using IGF1R-Beta mouse mAb against truncated IGF1R recombinant protein.
Immunohistochemistry: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052]

Immunohistochemistry: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052]

Immunohistochemistry: IGF-I R/IGF1R Antibody (3G5C1) [NB110-87052] - Expression of growth factor receptors in male breast cancer. Expression of HER2, FGFR2, EGFR, IGF-I R/IGF1R, and MET in representative cases of male breast cancer. Size bars equal 20 um. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0053353) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052]

Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052]

Immunohistochemistry-Paraffin: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis of ovarian cancer tissues using IGF1R-Beta mouse mAb with DAB staining.
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Applications for IGF-I R/IGF1R Antibody (3G5C1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 2 reviews rated 4.5 using NB110-87052 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IGF-I R/IGF1R

IGF1 Receptor (insulin-like growth factor 1 receptor, IGF1R), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF1 and IGF2, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation. Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.

Long Name

Insulin-like Growth Factor I Receptor

Alternate Names

CD221, IGF-1R, IGF-IR, IGF1R, IGFIR

Entrez Gene IDs

3480 (Human)

Gene Symbol

IGF1R

UniProt

P08069

Additional IGF-I R/IGF1R Products

Product Documents for IGF-I R/IGF1R Antibody (3G5C1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IGF-I R/IGF1R Antibody (3G5C1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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