IGF-II R/IGF2R Antibody (4J4A3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16270

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16270-100ul
NBP3-16270-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4J4A3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IGF-II R/IGF2R Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 2392-2491 of human IGF-II R/IGF2R (NP_000867.3). TTKSVKALSSLHGDDQDSEDEVLTIPEVKVHSGRGAGAESSHPVRNAQSNALQEREDDRVGLVRGEKARKGKSSSAQQKTVSSTKLVSFHDDSDEDLLHI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IGF-II R/IGF2R Antibody (4J4A3)

Western Blot: IGF-II R/IGF2R Antibody (4J4A3) [NBP3-16270]

Western Blot: IGF-II R/IGF2R Antibody (4J4A3) [NBP3-16270]

Western Blot: IGF-II R/IGF2R Antibody (4J4A3) [NBP3-16270] - Western blot analysis of extracts of A-549 cells, using Cation-independent IGF-II R/IGF2R (IGF2R) Rabbit mAb (NBP3-16270) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
IGF-II R/IGF2R Antibody (4J4A3)

Immunocytochemistry/Immunofluorescence: IGF-II R/IGF2R Antibody (4J4A3) [NBP3-16270] -

Immunocytochemistry/Immunofluorescence: IGF-II R/IGF2R Antibody (4J4A3) [NBP3-16270] - NIH/3T3 cells using Cation-independent M6PR (IGF2R) Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
IGF-II R/IGF2R Antibody (4J4A3)

Western Blot: IGF-II R/IGF2R Antibody (4J4A3) [NBP3-16270] -

Western Blot: IGF-II R/IGF2R Antibody (4J4A3) [NBP3-16270] - Analysis of extracts of Mouse heart, using Cation-independent Cation-independent M6PR (IGF2R) (IGF2R) Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for IGF-II R/IGF2R Antibody (4J4A3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IGF-II R/IGF2R

CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW].

Long Name

Insulin-like Growth Factor II Receptor

Alternate Names

CD222, CIMPR, IGF-IIR, IGF2R, IGFIIR, M6P-R, MPRI

Gene Symbol

IGF2R

Additional IGF-II R/IGF2R Products

Product Documents for IGF-II R/IGF2R Antibody (4J4A3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IGF-II R/IGF2R Antibody (4J4A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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