IGF-II R/IGF2R Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84598

Novus Biologicals, part of Bio-Techne
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NBP1-84598
NBP1-84598-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IGF-II R/IGF2R Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CKKDIFKANKEVPCYVFDEELRKHDLNPLIKLSGAYLVDDSDPDTSLFINVCRDIDTLRDPGSQLRACPPGTAACLVRGHQAFDVGQPRDGLKLVRKDRLVLSYVREEAGKLDFCDGHSPAVTITFVCPSE

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 20041192).

Marker

Late Endosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IGF-II R/IGF2R Antibody

Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody [NBP1-84598]

Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody [NBP1-84598]

Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NBP1-84598] - Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: IGF-II R/IGF2R Antibody [NBP1-84598]

Immunohistochemistry-Paraffin: IGF-II R/IGF2R Antibody [NBP1-84598]

Immunohistochemistry-Paraffin: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NBP1-84598] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Applications for IGF-II R/IGF2R Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. WB reported in the scientific literature (PMID: 20041192).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IGF-II R/IGF2R

CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW].

Long Name

Insulin-like Growth Factor II Receptor

Alternate Names

CD222, CIMPR, IGF-IIR, IGF2R, IGFIIR, M6P-R, MPRI

Gene Symbol

IGF2R

Additional IGF-II R/IGF2R Products

Product Documents for IGF-II R/IGF2R Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IGF-II R/IGF2R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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