IkB-alpha [p Ser36] Antibody (8G1X10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16912

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16912-100ul
NBP3-16912-20ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8G1X10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IkB-alpha Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S36 of human IkB-alpha (P25963). LDSMK

Modification

p Ser 36

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IkB-alpha [p Ser36] Antibody (8G1X10)

Western Blot: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Western blot analysis of extracts of various cell lines, using IkB-alpha Rabbit mAb (NBP3-16912) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight or treated by LPS (1 ug/mL) at 37C for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-alpha (20 ng/mL) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry of paraffin-embedded mouse liver using IkB-alpha Rabbit mAb (NBP3-16912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry of paraffin-embedded rat ovary using IkB-alpha Rabbit mAb (NBP3-16912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for IkB-alpha [p Ser36] Antibody (8G1X10)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

Additional IkB-alpha Products

Product Documents for IkB-alpha [p Ser36] Antibody (8G1X10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IkB-alpha [p Ser36] Antibody (8G1X10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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