IkB-alpha [p Tyr42] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23389
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
IkB-alpha (Tyr-42) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 42 of human IkB-alpha. This peptide sequence has low homology to other I?B proteins, but does have some homology to unrelated proteins that may have a conserved tyrosine phosphorylation motif. [UniProt# P25963]
Modification
p Tyr42
Specificity
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IkB-alpha (Tyr-42) peptide (without carrier). The antibody detects a 38 kDa protein on SDS-PAGE immunoblots of A431 and Jurkat cells treated with pervanadate, but not in control cells. Due to homologies to tyrosine sites on other proteins, it is recommended that the antibody be used to detect phosphorylation of immunoprecipitated IkB-alpha.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for IkB-alpha [p Tyr42] Antibody
![IkB-alpha [p Tyr42] Antibody](https://resources.bio-techne.com/images/products/nbp3-23389_rabbit-ikb-alpha-p-tyr42-pab-19920231856943.jpg "Western Blot: IkB-alpha [p Tyr42] Antibody [NBP3-23389] -")
Western Blot: IkB-alpha [p Tyr42] Antibody [NBP3-23389] -
Western Blot: IkB-alpha [p Tyr42] Antibody [NBP3-23389] - Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IkB-alpha (lane 1), anti-IkB-alpha (Tyr-42) (NBP3-23389; lanes 2-5), or anti-IkB-alpha (Tyr-305) (NBP3-23390; lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of IkB-alpha (Tyr-42) (lane 3 & 8) or IkB-alpha (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).Applications for IkB-alpha [p Tyr42] Antibody
Application
Recommended Usage
Immunoprecipitation
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: IkB-alpha
Long Name
I-kappa-B-alpha
Alternate Names
IkBalpha, MAD-3, NFKBIA
Gene Symbol
NFKBIA
Additional IkB-alpha Products
Product Documents for IkB-alpha [p Tyr42] Antibody
Product Specific Notices for IkB-alpha [p Tyr42] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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