Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Primate

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6H6

Format

BSA Free

Concentration

0.5 mg/ml

View all IL-3R alpha Antibodies »

Product Specifications

Immunogen

Tissue / cell preparation. IL3R transfected COS cells.

Localization

Type I membrane protein.

Specificity

IL3RA (6H6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IL-3R alpha/CD123 Antibody (6H6) - BSA Free

Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]

Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Analysis using the PE/Cy7 conjugate of NB600-1185. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy7 (NBP1-42944) (open histogram) or Anti-Human CD123 PE-Cy7 (filled histogram). Cells in the lymphocyte gate were
Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]

Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Staining of TF-1 cells with Purified Mouse IgG1, K Iso Cntrl (open histogram) or Purified anti-human CD123 (6H6) (colored histogram) followed by PE Anti-Mouse IgG. Total viable cells were used for analysis
Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]

Flow Cytometry: IL-3R alpha/CD123 Antibody (6H6) - BSA Free [NB600-1185]

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy7 (NBP1-42944) (open histogram) or Anti-Human CD123 PE-Cy7 (filled histogram). Cells in the lymphocyte gate were used for analysis.
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Applications for IL-3R alpha/CD123 Antibody (6H6) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1:10 - 1:1000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Flow Cytometry Use 1ug for 10^6 cells in 100 ul volume or 100 ul of whole blood. This antibody has been tested by tested by immunofluorescent staining with flow cytometric analysis. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Although not tested this antibody may be useful in IHC-P and Immunoprecipitation. WB was reported in the scientific literature (PMID:12576313).

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IL-3R alpha

IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Entrez Gene IDs

3563 (Human)

Gene Symbol

IL3RA

Additional IL-3R alpha Products

Product Documents for IL-3R alpha/CD123 Antibody (6H6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IL-3R alpha/CD123 Antibody (6H6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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